Aining in situ synthesized 60-mers was produced by Agilent Technologie…

Aining in situ synthesized 60-mers was produced by Agilent Technologies Rosiglitazone (Santa Clara, USA), according to a custom probe design based on the genome sequence of S. suis P1/7 [7]. A total of 7651 unique 60-mers having a theoretical melting temperature of approximately 81 and representing 1960 ORFs were selected as described by Saulnier et al. [28]. Genes were represented by 4 (91 ), 3 (4 ), 2 (2 ), or 1 probe (3 ). A total of 25 putative genes were not represented on the array because no unique probe satisfying the selection criteria could be selected.Comparative genome hybridization (CGH)Chromosomal DNA (50 g) was sheared in 1 ml shearing buffer (TE/10 glycerol), using Nebulizers (Invitrogen, Carlsbad, USA) under 1.7 bar air pressure for 3 minutes to yield fragments between 500 and 1500 bp. DNA was ethanol precipitated, taken up in water and 10 g of DNA wasde Greeff et al. BMC Microbiology 2011, 11:161 http://www.biomedcentral.com/1471-2180/11/Page 3 ofTable 1 Characteristics of bacterial strains used in this studyStrain 6388 6112 NCTC10273R1 C160 C187 OV585 12 16 25 T15 89-1591 3995 3988 17 S735R2 1890 3 10 22 D282 7696 P1/7 BM190 BM191 BM334 BM407 FX59 FX125 95-8242 89-999 R75/S2 98HAH12 05ZYH33 OV233 OV349 OV625 OV209 7711 7917 8039 C126 15009 A496/98 Sw123/B2452 Serotype Virulence* ST 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 4 7 7 7 7 7 7 7 HV HV V ND ND ND AV AV AV AV ND ND ND WV WV ND V V V V ND V ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 1 1 13 1 132 13 19 19 20 19 25 1 1 8 1 133 1 1 1 1 1 1 1 1 1 1 1 28 1 25 1 7 7 1 1 1 17 29 29 135 1 89 29 29 MRP# EF# SLY# S S + + + + + + + + + + + + + + + + + + + + + + + S + + + + * * * * * + + + + + + * * + * + + + + + + + + + + + + + + + +/+ + + + + + + + + + + + + + + + + + + + + + + Clinical source Organs Organs Unknown Brain/septicemia Tonsil/septicemia Unknown Tonsil Tonsil Human Tonsil Unknown Unknown Unknown Tonsil Unknown Unknown CNS Tonsil Human CNS Joint Unknown Human sepsis, meningitis Reference/source Laboratory collection [32] Laboratory collection [32] Laboratory collection [32] Laboratory collection [9] Laboratory collection [9] Veterinary Practice Diessen, The Netherlands Laboratory collection [21] Laboratory collection [21] Laboratory collection [21] Laboratory collection [23] Groupe de Recherch?sur les Maladies Infectieuses du Porc, Qu ec, Canada, [56] Laboratory collection [16] Laboratory collection Laboratory collection Laboratory collection CVI Laboratory collection Laboratory collection [21] Laboratory collection [21] Laboratory collection [21] Laboratory collection [21] Groupe de Recherceh sur les Maladies Infectieuses du Porc, Qu ec, Canada Laboratory collection CVI Hospital for Tropical Diseases, Ho Chi Minh City, VietnamHuman meningitis Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam Human meningitis Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam Human meningitis Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam tonsil tonsil Unknown Unknown Unknown Human TSLS Human TSLS Unknown Unknown Unknown Unknown CNS CNS CNS Joint Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam Hospital for Tropical Diseases, Ho PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/17139194 Chi Minh City, Vietnam Groupe de Recherceh sur les Maladies Infectieuses du Porc, Qu ec, Canada Groupe de Recherceh sur les Maladies Infectieuses du Porc, Qu ec, Canada, [56] Groupe de Recherceh sur les Maladies Infectieuses du Porc, Qu ec, Canada, [56] [41]Hua Dong Research In.